反應(yīng)種屬：Candida albicans, Chinese Hamster, Dog, Drosophila, E. coli, Hamster, Human, Monkey, Mouse, Pig, Rat, Yeast, Zebrafish
宿主來源：Mouse
實(shí)驗(yàn)應(yīng)用：EMSA, ICC/IF, IHC-P, WB
同種型：IgG2b
免疫原：Recombinant protein encompassing a sequence within the center region of human GAPDH. The exact sequence is proprietary.
克隆性：Monoclonal
克隆號：GT239
純化方式：Affinity purified by Protein G.
偶聯(lián)：Unconjugated
產(chǎn)品濃度：1 mg/ml (Please refer to the vial label for the specific concentration.)
保存溫度：Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4oC. For long-term storage, aliquot and store at -20oC or below. Avoid multiple freeze-thaw cycles.
運(yùn)輸溫度：4°C
預(yù)期分子量：36 kDa
產(chǎn)品形式：Liquid
存儲溶液：PBS, no preservative.
驗(yàn)證數(shù)據(jù)
WB Image
GAPDH antibody [GT239] detects GAPDH protein by western blot analysis.
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
ICC/IF：
GAPDH antibody [GT239] detects GAPDH protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: GAPDH stained by GAPDH antibody [GT239] (GTX627408) diluted at 1:500.
Blue: Hoechst 33342 staining.
IHC-P：
GAPDH antibody [GT239] detects GAPDH protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
GAPDH stained by GAPDH antibody [GT239] (GTX627408) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
產(chǎn)品部分引用文獻(xiàn)
1.S Shivaprasad et al. Viruses 2024; 16 (5) : 725 CRISPR Screen Reveals PACT as a Pro-Viral Factor for Dengue Viral Replication.
2.X Chen et al. Nature 2024; 628 (8009) : 818-825 Antisense oligonucleotide therapeutic approach for Timothy syndrome.
3.CP Chen et al. Heliyon 2024; 10 (7) : 29055 Synergistic effects of the combination of trametinib and alpelisib in anaplastic thyroid cancer with BRAF and PI3KCA co-mutations.
4.YL Chen et al. Viruses 2024; 16 : 523 Novel Anti-Viral Properties of the Herbal Extract of Davallia mariesii against Influenza A Virus.
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