保存溫度:Stable for six (6) months at -80°C from date of receipt. For best results, aliquot and avoid multiple freeze/thaws.
運(yùn)輸溫度:Dry ice.
產(chǎn)品形式:Purified recombinant mononucleosomes, (21.8 µg protein weight, 50 µg DNA+protein) in 44.2 µL of 10 mM Tris-HCl pH 7.5, 25 mM NaCl, 1 mM EDTA, 2 mM DTT, 20% glycerol. MW = 240,650 Da. Molarity = 4.7 µM.
驗(yàn)證數(shù)據(jù)
Figure 1: DNA Gel Data
EpiDyne-FRET substrate resolved via native PAGE gel and
stained with ethidium bromide to visualize DNA. Lane 1: Free
DNA (100 ng). Lane 2: Intact EpiDyne-FRET nucleosomes
(400 ng).
Figure 2: Protein Gel Data
Coomassie stained PAGE gel of proteins in EpiDyne-FRET
substrate (1 µg) demonstrates the purity of histones in the
preparation. Sizes of molecular weight markers and positions
of the core histones (H2A-Cy5, H2B, H3.2, and H4) are